Multi-omics quantitative data of tomato fruit unveils regulation modes of least variable metabolites

Annick Moing (1, 2) , Thierry Berton (1) , Léa Roch (1) , Salimata Diarrassouba (1, 3) , Stéphane Bernillon (4, 1, 2) , Stéphanie Arrivault (5) , Catherine Deborde (1, 6, 2) , Mickaël Maucourt (1, 2) , Cecile Cabasson (2, 1) , Camille Bénard (1) , Sylvain Prigent (1, 2) , Daniel Jacob (1, 2) , Yves Gibon (1, 2) , Martine Lemaire-Chamley (1)

1 BFP - Biologie du fruit et pathologie
2 Plateforme Bordeaux Metabolome
3 LRSV - Laboratoire de Recherche en Sciences Végétales
4 MycSA - Unité de recherche Mycologie et Sécurité des Aliments
5 MPI-MP - Max Planck Institute of Molecular Plant Physiology
6 BIBS - BioInformatique et BioStatistiques

Background The composition of ripe fruits depends on various metabolites which content evolves greatly throughout fruit development and may be influenced by the environment. The corresponding metabolism regulations have been widely described in tomato during fruit growth and ripening. However, the regulation of other metabolites that do not show large changes in content have scarcely been studied. Results We analysed the metabolites of tomato fruits collected on different trusses during fruit development, using complementary analytical strategies. We identified the 22 least variable metabolites, based on their coefficients of variation. We first verified that they had a limited functional link with the least variable proteins and transcripts. We then posited that metabolite contents could be stabilized through complex regulations and combined their data with the quantitative proteome or transcriptome data, using sparse partial-least-square analyses. This showed shared regulations between several metabolites, which interestingly remained linked to early fruit development. We also examined regulations in specific metabolites using correlations with individual proteins and transcripts, which revealed that a stable metabolite does not always correlate with proteins and transcripts of its known related pathways. Conclusions The regulation of the least variable metabolites was then interpreted regarding their roles as hubs in metabolic pathways or as signalling molecules.

Keywords: Fruit; LC-MS; Metabolism regulation; Proton NMR; Omics; Solanum lycopersicum

Domains: Life Sciences [q-bio]; Vegetal Biology

Annick Moing, Thierry Berton, Léa Roch, Salimata Diarrassouba, Stéphane Bernillon, et al.. Multi-omics quantitative data of tomato fruit unveils regulation modes of least variable metabolites. BMC Plant Biology, 2023, 23 (365), ⟨10.1186/s12870-023-04370-0⟩. ⟨hal-04183193⟩