A sedimented sample of a 59 kDa dodecameric helicase yields high-resolution solid-state NMR spectra.

Carole Gardiennet (1) , Anne K Schütz , Andreas Hunkeler , Britta Kunert (1) , Laurent Terradot (1) , Anja Böckmann (1) , Beat H Meier

1 IBCP - Institut de biologie et chimie des protéines [Lyon]

Crystal clear: Preparing solid-state NMR samples that yield high-resolution spectra displaying high sensitivity is time-consuming and complicated. A sample of the 59 kDa protein DnaB, prepared simply by preparative centrifugation, provides spectra that are as good as the ones from carefully grown microcrystals.

Domains: Life Sciences [q-bio]

Carole Gardiennet, Anne K Schütz, Andreas Hunkeler, Britta Kunert, Laurent Terradot, et al.. A sedimented sample of a 59 kDa dodecameric helicase yields high-resolution solid-state NMR spectra.. Angewandte Chemie International Edition, 2012, 51 (31), pp.7855-8. ⟨10.1002/anie.201200779⟩. ⟨hal-00965867⟩